Bezanilla Lab
Movies

 

 


Protonemal Growth


 
 

 


Wildtype protonemal cells grown on agar pad.
 


Profilin-RNAi
plant grown on agar pad.

 

 


Myosin VIII
regulates branch formation. Images were acquired with a stereo microscope. Plants were growing on cellophane overlaid PpNH4 medium.

 


Wild type cells expressing a nuclearly localized GFP:GUS fusion protein. Plants were imaged while growing on cellophane overlaid PpNH4 medium with a stereo microscope. Red fluorescence is from the chloroplasts.

Imaging Actin with Lifeact


 


F-actin imaged with Lifeact-mEGFP transiently transformed into a lily pollen tube. Images were acquired with a scanning confocal.

 


F-actin imaged with Lifeact-mEGFP transiently transformed into a tobacco pollen tube. Images were acquired with a scanning confocal.

 


F-actin imaged with Lifeact-mEGFP stably expressed in moss protonemata. Images were acquired with a scanning confocal.

 


Cortical F-actin imaged with Lifeact-mEGFP stably expressed in moss protonemata. Images were acquired with a TIRF microscope.

Actin dynamics at the cell cortex imaged with Lifeact-mEGFP in wild type cells.


F-actin translocation event
in wild type cells.


F-actin growth and shrinkage around bundled cluster

F-actin severing

Cortical F-actin dynamics are dramatically reduced in plants lacking actin severing proteins.


Cortical F-actin in ∆aip1 plants
imaged with Lifeact-mEGFP.
Images were acquired with a TIRF microscope.


Cortical F-actin in ADF-RNAi plants
imaged with Lifeact-mEGFP. Images were acquired with a spinning disc confocal microscope
Formin-mediated actin polymerization

 

 


For2A-3XmEGFP
(green) and Lifeact-mCherry (red) in growing wild type cells. Images were acquired with a scanning confocal.

 


For2A-3XmEGFP
(green) accumulations in the cytoplasm generate bursts of actin (red) polymerization.

 


For2A-3X-mEGFP
localizes to the cell
cortex as discrete dynamic spots. The localization to the cortex is independent of actin. But linear motility of these dots is dependent on actin. Images were acquired on a TIRF microscope.

 

 

   


For2A-3XmEGFP
(magenta) generates actin filaments (labeled with Lifeact-mCherry, cyan) de novo and along pre-existing actin filaments at the cell cortex. Images were acquired with a dual view TIRF system.

 


For2A-3XmEGFP
(magenta) generates actin filaments (labeled with Lifeact-mCherry, cyan) along pre-existing actin filaments at the cell cortex. Images were acquired with a dual view TIRF system.

   

Cell Division

 

 


GFP-tubulin
is used to image microtubules during mitosis in an apical cell. Images were acquired with a spinning disc confocal.

 


For2A-3XmEGFP
localizes to the apex of growing cells and to the phragmoplast during division. Images were acquired with a scanning confocal.

 


N-terminal PTEN
domain mediates For2A localization since PTEN-mEGFP also localizes to the apex of growing cells and to the phragmoplast during division. Images were acquired with a scanning confocal.